Trump has deleted some of the Twitter tirade he posted following Mr Obama's re-election and added new critiques of US TV network NBC's Brian Williams.
Trump started out in a philosophical mood as the tide turned against Republican candidate Mitt Romney, musing "Well, back to the drawing board."
But it appears it was all too much for the property tycoon as reality of Mr Obama's clear win began to sink in.
"We can’t let this happen. We should march on Washington and stop this travesty. Our nation is totally divided!”, Trump fired off on Twitter.
The Apprentice star, who toyed with a Republican presidential run, became increasingly irate, even calling for Americans to reject Mr Obama’s resounding victory.
"Let’s fight like hell and stop this great and disgusting injustice! The world is laughing at us. This election is a total sham and a travesty. We are not a democracy."
The US "now in serious and unprecedented trouble…like never before", Trump tweeted, adding: "the electoral college is a disaster for democracy.”
Williams showed some of Trump's tweets on air, saying he had "driven well past the last exit to relevance and veered into something closer to irresponsible."
Trump tweeted a retort today, saying Williams "knows that I think his newscast has become totally boring so he took a shot at me last night." He added: "Wouldn't you love to have my ratings?"
He also deleted some of Tuesday night's tirade, including tweets calling for revolution and incorrectly saying that Mr Obama had lost the popular vote. In a new tweet today, he said the election was "the Republicans to win" but that Mr Romney hadn't connected with the people.
Obama supporter and 30 Rock star Alec Baldwin was quick to return fire.
"You trust the voters when they choose The Apprentice. But not now?", Baldwin tweeted.
Trump has long been an outspoken critic of Mr Obama as a key figure in the "birther" movement, pressuring Mr Obama to release his birth certificate and prove he was born in the US and thus eligible for the White House. He managed to stay in the pubic eye with his recent claims of a "bombshell" about Mr Obama, which turned out to be a bizarre $5 million charity offer if Mr Obama would release his university transcript.
Appearing on The Tonight Show with Jay Leno during the campaign, Mr Obama said he had never met Trump, but joked that the businessman's animosity dated back to their childhood soccer rivalry "in Kenya".