After 50 years of spinning webs and catching criminals Peter Parker, the alter ego of Spider-Man, has met his doom.
In the 700th and final issue of "Amazing Spider-Man" the character is killed off in a battle with his nemesis Otto Octavius, also known as Dr Octopus.
The plot sees Parker's mind trapped in the withered and dying body of Octavius, who in turn occupies the body of the superhero.
Dan Slott, who writes the Spider-Man adventures for Marvel Entertainment, described it as an "epic turn."
The move has caused controversy among fans and Mr Slott said he may have to go into hiding.
He told the Los Angeles Times: "My exact phrase was, 'I'm pulling a Salman Rushdie'. Peter Parker means a lot to people. Not just comic book fans, but fans of the movies, cartoons, toys and even the Thanksgiving Day balloon. He's been with us for over half a century.
"It was tough to do this. I've been a Spider-Man fan since age eight. Short of my father, this guy's been my lifelong hero."
The demise of Peter Parker and "Amazing Spider-Man" will see the start of a new series called "The Superior Spider-Man" following Octavius.
Editor Stephen Wacker said: "His (Parker's) life is still important because it affects everything that Doctor Octopus does as Spider-Man. Seeing a supervillain go through this life is the point, trying to be better than the hero he opposed."